EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.1.1.112 | 1.02 |
- |
oxaloacetate |
mutant enzyme H235A, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C |
678331 |
| 4.1.1.112 | 1.39 |
- |
oxaloacetate |
in 100 mM Tris-HCl (pH 7.3), 10 mM MgCl2, 2.5 mM EDTA, at 30°C |
704327 |
| 4.1.1.112 | 1.7 |
- |
oxaloacetate |
- |
4283 |
| 4.1.1.112 | 2.1 |
- |
oxaloacetate |
- |
4276 |
| 4.1.1.112 | 2.1 |
- |
oxaloacetate |
pH 8.0, 25°C |
654328 |
| 4.1.1.112 | 2.2 |
- |
oxaloacetate |
wild type enzyme, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C |
678331 |
| 4.1.1.112 | 2.5 |
- |
oxaloacetate |
mutant enzyme H235Q, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C |
678331 |
| 4.1.1.112 | 3.3 |
- |
pyruvate |
- |
4263 |
| 4.1.1.112 | 4.3 |
- |
oxaloacetate |
- |
4272 |
| 4.1.1.112 | 20.7 |
- |
oxaloacetate |
pH 7.4, 25°C, wild-type enzyme |
756006 |
