EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    2.6.1.16 | 0.1 |
- |
L-gamma-glutamyl-p-nitroanilide |
mutant W74L, 37°C |
673687 |
| 2.6.1.16 | 0.1 |
- |
L-glutamine |
mutant A602L, 37°C |
673687 |
| 2.6.1.16 | 0.1 |
- |
L-glutamine |
mutant enzyme A602L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C |
673687 |
| 2.6.1.16 | 0.11 |
- |
L-glutamine |
synthase activity, pH 7.2, 37°C |
737433 |
| 2.6.1.16 | 0.14 |
- |
L-glutamine |
hemisynthase activity, pH 7.2, 37°C |
737433 |
| 2.6.1.16 | 0.17 |
- |
L-glutamine |
wild type enzyme |
672444 |
| 2.6.1.16 | 0.2 |
- |
D-fructose 6-phosphate |
wild type enzyme |
672444 |
| 2.6.1.16 | 0.2 |
- |
L-gamma-glutamyl-p-nitroanilide |
mutant W74A, 37°C |
673687 |
| 2.6.1.16 | 0.2 |
- |
L-glutamate |
calorimetric determination, pH 7.2, 25°C |
721419 |
| 2.6.1.16 | 0.2 |
- |
L-glutamine |
pH 7.4, 37°C |
639920 |
