EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.4.2.29 | 0.00051 |
- |
tRNA |
histidine-tagged mutant enzyme D89E, pH 8.5 |
644933 |
2.4.2.29 | 0.00632 |
- |
guanine |
histidine-tagged mutant enzyme D89E, pH 8.5 |
644933 |
2.4.2.29 | 0.0001 |
- |
guanine |
histidine-tagged wild-type enzyme, pH 7.3 |
644933 |
2.4.2.29 | 0.00012 |
- |
tRNA |
histidine-tagged wild-type enzyme, pH 7.3 |
644933 |
2.4.2.29 | 0.00039 |
- |
tRNA |
histidine-tagged wild-type enzyme, pH 8.5 |
644933 |
2.4.2.29 | 0.00057 |
- |
guanine |
histidine-tagged wild-type enzyme, pH 8.5 |
644933 |
2.4.2.29 | -999 |
- |
more |
kinetic parameters of recombinant and native enzyme |
728740 |
2.4.2.29 | 0.001 |
- |
tRNA guanine |
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |
2.4.2.29 | 0.0033 |
- |
guanine |
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |
2.4.2.29 | 0.032 |
- |
pre-queuine 1 |
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |