EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.4.2.29 | 0.00039 |
- |
tRNA |
histidine-tagged wild-type enzyme, pH 8.5 |
644933 |
2.4.2.29 | 0.0005 |
- |
pre-queuine 0 |
mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |
2.4.2.29 | 0.00051 |
- |
tRNA |
histidine-tagged mutant enzyme D89E, pH 8.5 |
644933 |
2.4.2.29 | 0.00057 |
- |
guanine |
histidine-tagged wild-type enzyme, pH 8.5 |
644933 |
2.4.2.29 | 0.0007 |
- |
pre-queuine 1 |
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |
2.4.2.29 | 0.00087 |
- |
virF minihelix RNA |
at pH 7.3 |
689250 |
2.4.2.29 | 0.0009 |
- |
pre-queuine 0 |
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |
2.4.2.29 | 0.0009 |
- |
tRNA guanine |
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |
2.4.2.29 | 0.00098 |
- |
[tRNATyr]7-aminomethyl-7-carbaguanine |
mutant K52M |
705164 |
2.4.2.29 | 0.001 |
- |
tRNA guanine |
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 |
688390 |