EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   2.1.1.56 | 0.183 |
- |
G(5')pppR-RNA |
mutant enzyme R632Q, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.2 |
- |
G(5')pppR-RNA |
mutant enzyme K607Q, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.218 |
- |
G(5')pppR-RNA |
mutant enzyme E763A, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.24 |
- |
GpppA |
- |
674701 |
| 2.1.1.56 | 0.255 |
- |
G(5')pppR-RNA |
mutant enzyme F679H, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.293 |
- |
G(5')pppR-RNA |
mutant enzyme F679V, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.314 |
- |
G(5')pppR-RNA |
mutant enzyme K607R, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.446 |
- |
G(5')pppR-RNA |
mutant enzyme N570D, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 1 |
- |
GTP |
- |
674439 |
| 2.1.1.56 | 1.8 |
- |
G(5')pppR-RNA |
pH 7.5, 20°C, Michaelis constants for S-adenosyl-methionine (AdoMet) in both reactions are about 10fold lower than for their respective RNA substrates, suggesting that the rate-limiting steps in methylase reactions are associated with RNA templates |
721099 |
