EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.8.4.9 | 0.0112 |
- |
5'-adenylyl sulfate |
30°C, pH 8.5, cosubstrate: dithiothreitol |
689431 |
| 1.8.4.9 | 0.0122 |
- |
5'-adenylylsulfate |
with 40 mM glutathione as reductant |
288644 |
| 1.8.4.9 | 0.0174 |
- |
5'-adenylyl sulfate |
30°C, pH 8.5, cosubstrate: glutathione |
689431 |
| 1.8.4.9 | 0.02 |
- |
3'-phosphoadenosine 5'-phosphosulfate |
- |
687585 |
| 1.8.4.9 | 0.031 |
- |
3'-phosphoadenosine-5'-phosphosulfate |
assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C |
667703 |
| 1.8.4.9 | 0.05 |
- |
adenosine 5'-phosphosulfate |
- |
687585 |
| 1.8.4.9 | 0.4 |
- |
DTT |
with 5'-adenylylsulfate as substrate |
288642 |
| 1.8.4.9 | 0.495 |
- |
glutathione |
30°C, pH 8.5 |
689431 |
| 1.8.4.9 | 0.595 |
- |
dithiothreitol |
30°C, pH 8.5 |
689431 |
| 1.8.4.9 | 0.6 |
- |
glutathione |
holoenzyme APR1p with substrate 5'-adenylyl sulfate, pH 8.5, 30°C |
660399 |
