EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.3.98.1 | 0.091 |
- |
L-dihydroorotate |
pH 8.0, 22°C, wild-type |
656285 |
1.3.98.1 | 0.0975 |
- |
fumarate |
in the presence of 0.03 mM dihydroorotate |
676964 |
1.3.98.1 | 0.105 |
- |
L-dihydroorotate |
pH 8.0, 22°C, mutant R265A |
656285 |
1.3.98.1 | 0.115 |
- |
2,6-dichloroindophenol |
in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C |
686821 |
1.3.98.1 | 0.12 |
- |
fumarate |
assay based on isothermal titration calorimetry, pH 8.0, 25°C |
710846 |
1.3.98.1 | 0.12 |
- |
fumarate |
pH not specified in the publication, temperature not specified in the publication |
710846 |
1.3.98.1 | 0.123 |
- |
L-dihydroorotate |
pH 8.0, 22°C, mutant H185A |
656285 |
1.3.98.1 | 0.1282 |
- |
fumarate |
in the presence of 0.04 mM dihydroorotate |
676964 |
1.3.98.1 | 0.344 |
- |
ferricyanide |
pH 8.5, 70°C |
655517 |
1.3.98.1 | 0.4135 |
- |
fumarate |
in the presence of 0.05 mM dihydroorotate |
676964 |