EC Number   |
KM Value [mM]  |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.17.1.9 | 29.5 |
- |
NADP+ |
in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C |
696475 |
| 1.17.1.9 | 29.8 |
- |
formate |
mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication |
740027 |
| 1.17.1.9 | 30 |
- |
formate |
with 3-pyridinecarboxaldehyde-NAD+ as electron acceptor |
288085 |
| 1.17.1.9 | 30 |
- |
NAD+ |
mutant enzyme K328V, at 20°C, after 4 months of enzyme storage |
689904 |
| 1.17.1.9 | 30 |
50 |
CO2 |
- |
288092 |
| 1.17.1.9 | 31 |
- |
formate |
mutant E141Q, 30°C, pH 7.0 |
669327 |
| 1.17.1.9 | 32 |
- |
CO2 |
mutant enzyme D221S, pH and temperature not specified in the publication |
741547 |
| 1.17.1.9 | 35 |
- |
formate |
native wild type enzyme, at 20°C, after 4 months of enzyme storage |
689904 |
| 1.17.1.9 | 37 |
- |
NADP+ |
at pH 7.0 and 25°C |
763261 |
| 1.17.1.9 | 38 |
- |
NADP+ |
Km above 38 mM, wild type enzyme, in 0.1 M potassium phosphate buffer (pH 7.5) |
686705 |
