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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
1.10.3.3
0.98
-
L-ascorbate
Warburg method
439924
1.10.3.3
1.12
-
5,6-ene-L-ascorbate
commercial enzyme preparation from Sigma
439931
1.10.3.3
1.125
-
L-ascorbate
Warburg method
439924
1.10.3.3
1.307
-
D-isoascorbate
commercial enzyme preparation from Sigma
439931
1.10.3.3
1.5
-
L-ascorbic acid
-
439928
1.10.3.3
1.89
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.4, 30°C
764908
1.10.3.3
2
-
L-ascorbate
manomeric assay following the rate of O2 uptake
439889
1.10.3.3
5
-
L-ascorbate
manomeric assay following the rate of O2 uptake
439889
1.10.3.3
10
-
L-ascorbate
enzyme from soluble fraction and cell-wall preparation
439893
Results
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