EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.1.1.87 | 0.58 |
- |
isocitrate |
mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.59 |
- |
(2R,3S)-3-(2-hydroxyethyl)malate |
pH 7.8, 28°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.6 |
- |
ethylmalate |
pH 7.8, 36°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.6 |
- |
NAD+ |
mutant enzyme L81P, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.62 |
- |
NAD+ |
mutant enzyme L83R, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.64 |
- |
NAD+ |
mutant enzyme I82N, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.68 |
- |
3-isopropylmalate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.71 |
- |
3-isopropylmalate |
mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.76 |
- |
NAD+ |
mutant enzyme S80A, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.78 |
- |
(2R,3S)-3-propylmalic acid |
pH 7.8, 28°C, recombinant enzyme |
667934 |