EC Number   |
Inhibitors |
Structure |
|---|
   3.5.1.93 | (7R)-7-(4-carboxybutanamido)cephalosporanate |
inhibition of mutant M165alphaS/H57betaS/H70betaS, no inhibition of wild-type enzyme and mutant H57betaS/H70betaS |
   |
| 3.5.1.93 | (7R)-7-(4-carboxybutanamido)cephalosporanate |
substrate inhibition |
|
| 3.5.1.93 | (7R)-7-aminocephalosporanate |
product inhibition |
|
| 3.5.1.93 | 6-aminopenicillanic acid |
6-APA, a noncompetitive inhibitor |
|
| 3.5.1.93 | 7-aminocephalosporanate |
20 mM, 81% inhibition |
|
| 3.5.1.93 | 7-aminocephalosporanate |
competitive |
|
| 3.5.1.93 | 7-aminocephalosporanic acid |
product inhibition, competitive inhibitor |
|
| 3.5.1.93 | 7beta-3-bromopropionyl aminocephalosporanic acid |
Trp4 of the beta-subunit is alkylated |
|
| 3.5.1.93 | 7beta-bromoacetyl amino cephalosporanic acid |
inhibits and alkylates the enzyme. The enzyme labeled with 7beta-bromoacetyl amino cephalosporanic acid is inactive at room temperature, but in the process of crystallization at 18°C it catalyzes the hydrolysis of 7beta-bromoacetyl amino cephalosporanic acid.. In crystals, 7-aminocephalosporanic acid is released but the acetic acid still binds with Trp-beta4, and as a result, the enzyme remains inactive |
|
| 3.5.1.93 | 7beta-bromoacetyl aminocephalosporanic acid |
Trp4 of the beta-subunit is alkylated |
|
