EC Number   |
Inhibitors |
Structure |
|---|
   3.4.22.B79 | (5E)-6-(4-methoxyphenyl)-4-oxo-N-(2-phenylethyl)hex-5-enamide |
topographical peptidomimetic, binds covalently leading to permanent enzyme inactivation via Michael adduct formation between the alpha/beta-unsaturated ketone functionality and the active site. 100% inhibition at 0.059 mg/ml, EC50 for cell-based assay 0.0089 mg/ml |
   |
| 3.4.22.B79 | (5E)-N-benzyl-4-oxo-6-phenylhex-5-enamide |
topographical peptidomimetic, binds covalently leading to permanent enzyme inactivation via Michael adduct formation between the alpha/beta-unsaturated ketone functionality and the active site. 100% inhibition at 0.068 mg/ml, EC50 for cell-based assay 0.0088 mg/ml |
|
| 3.4.22.B79 | Cu2+ |
strong inhibition with substrate 2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) |
|
| 3.4.22.B79 | Cu2+ |
- |
|
| 3.4.22.B79 | E64d |
inhibitor binds beneath a beta-hairpin at the interface of the SAM MTase and protease domains |
|
| 3.4.22.B79 | EDTA |
inhibition of nsp2pro at or above 2 mM EDTA |
|
| 3.4.22.B79 | glycerol |
activity is reduced by approximately 20fold in the presence of 5% glycerol |
|
| 3.4.22.B79 | Hg2+ |
- |
|
| 3.4.22.B79 | Mg2+ |
stimulation with substrates 2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) and 2-(N-methylamino)benzoyl-AGGGIIETk(Dnp), inhibition with substrate 2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) |
|
| 3.4.22.B79 | more |
dithiothreitol and tris (2-carboxyethyl) phosphine hydrochloride have no discernable effect on the activity up to 5 mM. The enzyme is able to tolerate up to 10 mM of beta-mercaptoethanol |
|
