EC Number   |
Inhibitors |
Structure |
|---|
   3.1.25.1 | Ca2+ |
inhibits both Py-Py correndonucleases |
   |
| 3.1.25.1 | CH2O |
total inhibition if 4.4 methylations per molecule are present |
|
| 3.1.25.1 | EDTA |
inhibits Py-Py correndonuclease II at concentrations above 20 mM |
|
| 3.1.25.1 | KCl |
Py-Py correndonuclease II loses 75% of activity in presence of 100 mM |
|
| 3.1.25.1 | Mg2+ |
in presence of 10 mM MgCl2: 10% of the activity observed in presence of 10 mM EDTA |
|
| 3.1.25.1 | Mg2+ |
Py-Py correndonuclease II loses 75% of activity in presence of 15 mM |
|
| 3.1.25.1 | Mg2+ |
inhibits the enzyme activity with DNA containing apurinic/apyrimidinic sites, but not with UV-radiation damaged DNA |
|
| 3.1.25.1 | more |
endonuclease activity not affected by: -SH-group inhibitors, caffeine, tRNA |
|
| 3.1.25.1 | more |
the enzyme is sensitive to high salt concentrations |
|
| 3.1.25.1 | NaBH4 |
inhibits degradation of the glycosylase product, reduces aldehyde moiety of the apyrimidinic sites to alcohol which results in resistance to endonuclease |
|
