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2.8.3.22
Borohydride
no inactivation by 0.1 mM borohydride is recorded in the test with and without succinyl-CoA, and nearly 85% of the activity is retained when the enzyme is preincubated with 10 mM hydroxylamine for 10 min in the presence of 2 mM succinyl-CoA. This suggests that no reactive enzyme-CoA intermediate is present. Inactivation with 10 mM borohydride decreases activity to 28% in the presence of 2 mM succinyl-CoA
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