2.3.1.304 | HBCH2CoA |
substrate analogue, in which the S-atom in hydroxybutanoyl-CoA is replaced with a CH2 group. HBCH2CoA is a competitive inhibitor of class I and class III PHB synthases. Upon incubation with a synthase acylated with a [3H]-saturated trimer-CoA, i.e. sTCoA, products are the methylene analogue of [3H]-sTCoA, [3H]-sT-CH2-CoA, saturated dimer-[3H]-sD-CO2H, and trimer-acid [3H]-sT-CO2H. HBCH2CoA may be reporting on the termination and repriming process of the synthases, rather than elongation |
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