EC Number   |
Inhibitors |
Structure |
|---|
   2.3.1.165 | (2S,3R)-2,3-epoxy-4-oxo-7,10-dodecadienoylamide |
mycotoxin produced by Cephalosporium caerulens, trivial name cerulenin, 0.2 mM, complete inactivation after approx. 30 min, second-order rate constant for the reaction with 6-methylsalicylic acid synthase: 13.8 M/s, acetyl-CoA protects, site of modification: Cys204 |
   |
| 2.3.1.165 | (2S,3R)-2,3-epoxy-4-oxo-7,10-dodecadienoylamide |
0.0014 mM, complete inactivation after 40 min, acetyl-CoA protects |
|
| 2.3.1.165 | 1,3-dibromo-propane-2-one |
0.02 mM, complete inactivation after 40 min, acetyl-CoA protects |
|
| 2.3.1.165 | 1,3-dibromo-propane-2-one |
very rapid inactivation, inactivation half-life: 7 s |
|
| 2.3.1.165 | 5,5'-dithiobis(2-nitrobenzoic acid) |
0.01 mM, rapid inactivation, inactivation half-life: approx. 15 s, both acetyl-CoA and malonyl-CoA protect |
|
| 2.3.1.165 | iodoacetamide |
0.5 mM, complete inactivation of synthetase activity after 15 min at 0°C, enzyme is then able to decarboxlate malonyl-CoA |
|
| 2.3.1.165 | iodoacetamide |
100 mM, complete inactivation after 45 min, site of modification: Cys204 |
|
| 2.3.1.165 | iodoacetamide |
100fold molar excess, complete inactivation after 30 min |
|
| 2.3.1.165 | more |
no inhibition by phenylmethylsulfonyl fluoride, a serine protease-type thioesterase inhibitor at 1 mM |
|
| 2.3.1.165 | NAD+ |
0.3 mM, 21% inhibition in crude extracts |
|
