EC Number   |
Inhibitors |
Structure |
|---|
   1.1.1.67 | ADP |
- |
   |
| 1.1.1.67 | AMP |
- |
|
| 1.1.1.67 | citrate |
50 mM, pH 5.4, 72% inhibition |
|
| 1.1.1.67 | Cu2+ |
1 mM, 61% inhibition |
|
| 1.1.1.67 | Cu2+ |
about 20% residual activity at 1 mM |
|
| 1.1.1.67 | CuSO4 |
1 mM, 28% inhibition of D-fructose reduction, 18% inhibition of D-sorbitol oxidation |
|
| 1.1.1.67 | D-fructose |
substrate inhibition, Ki: 1.356 mM |
|
| 1.1.1.67 | D-mannitol |
product inhibition, Ki: 24.6 mM |
|
| 1.1.1.67 | EDTA |
20 mM, complete inhibition, can be restored by 20 mM Zn2+, but not by Mn2+ or Mg2+ |
|
| 1.1.1.67 | EDTA |
activity of MtDH pretreated with 10 mM EDTA for 20 min at 37°C decreases 96%. Adding 20 mM ZnCl2 to the EDTA-treated enzyme restores activity up to 80% of the control (enzyme not treated with EDTA). Adding 20 mM CoCl2 and 20 mM MnCl2 to the EDTA-treated enzyme restores activity up to 132% and 94% of the control, respectively. In contrast, adding 20 mM MgCl2 or CaCl2 does not increase the activity of the EDTA-treated enzyme.; activity of MtDH pretreated with 10 mM for 20 min at 37°C decreases 96%. Adding 20 mM ZnCl2 to the EDTA-treated enzyme restores activity up to 80% of the control. Adding 20 mM CoCl2 and 20 mM MnCl2 to the EDTA-treated enzyme restores activity up to 132% and 94% of the control, respectively |
|
