EC Number   |
Inhibitors |
Structure |
|---|
    1.1.1.22 | ADP |
- |
   |
| 1.1.1.22 | AMP |
- |
|
| 1.1.1.22 | Ca2+ |
84% residual activity at 1 mM |
|
| 1.1.1.22 | EDTA |
83% residual activity at 1 mM |
|
| 1.1.1.22 | gallic acid |
is a non-competitive inhibitor with respect to UDP-glucose and NAD+. It decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Gallic acid exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of gallic acid, the activity remaining after 30 min is 55% that of control |
|
| 1.1.1.22 | Mn2+ |
84% residual activity at 1 mM |
|
| 1.1.1.22 | more |
no substrate inhibition, K+ is not inhibitory up to 500 mM, not inhibitory: dimethyldicarbonate, EDTA |
|
| 1.1.1.22 | more |
in the presence of 0.12 mM 5-azido-UDP-glucose, 89% of the enzyme activity is lost after 5 min of photolabeling. When the enzyme is photolyzed in presence of 1 mM UDP-glucose, 11% of the enzyme activity is lost. 5-azido-UDP-glucose is photoinserting into a UDP-glucose-binding site on the human enzyme in a specific manner. Uracil, uridine, and glucose have a poor protective effect on the labeling, while UDP and UDP-glucose strongly inhibit photoinsertion |
|
| 1.1.1.22 | more |
inhibition of human UDP-glucose dehydrogenase expression using siRNA expression vector in breast cancer cells |
|
| 1.1.1.22 | more |
the enzyme from serotype K30 is not inhibited by NAD+, in contrast to Escherichia coli K-12 |
|
