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Inhibitors
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acetoacetyl-CoA (0.2 mM), a potential inhibitor of enoyl-CoA hydratase, has no impact on enzyme activity of BoxCmal. Crotonyl-CoA (0.2 mM), a potential substrate of enoyl-CoA hydratase (crotonase), is neither converted to 3-hydroxybutyryl-CoA nor does it inhibit the standard assay
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