EC Number |
Inhibitors |
Structure |
---|
3.4.21.9 | more |
EDTA not inhibitory |
|
3.4.21.9 | Ca2+ |
slight inhibition of substrates with one or two Asp/Glu residues at P2-P3 positions |
|
3.4.21.9 | Ca2+ |
50% inhibition at 10 mM |
|
3.4.21.9 | NaCl |
at 100 and 250 mM, Gly-Asp-Asp-Asp-Asp-Lys-?-naphthylamide as substrate |
|
3.4.21.9 | dithiothreitol |
10% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate |
|
3.4.21.9 | Triton X-100 |
30-80% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate |
|
3.4.21.9 | ethanol |
80-40% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate |
|
3.4.21.9 | Urea |
50-70% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate |
|
3.4.21.9 | Urea |
use of higher amounts of urea (ca. 5 M) has an inhibitory effect on the enzyme activity |
|
3.4.21.9 | SDS |
40-70% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate |
|