EC Number |
Inhibitors |
Structure |
---|
1.13.11.60 | more |
not inhibited by 1 mM KCN. CO does not inhibit the enzyme |
|
1.13.11.60 | more |
catalysis declines due to suicide inactivation; catalysis declines due to suicide inactivation. Following column chromatography, e.g. ion exchange or zinc-affinity chromatography, hydroperoxide isomerase activity is often decreased and sometimes even undetectable by TLC, whereas 8-dioxygenase activity is always present. However, storage of the enzyme for a few days on ice often restores the hydroperoxide isomerase and appeares to increase the 7,8-linoleate diol synthase activity as well |
|
1.13.11.60 | more |
GSH (1 mM), GSSG (1 mM), EDTA (1 or 10 mM), EGTA (1 or 10 mM) and H202 (10 mM) are without effect on the 8R-dioxygenase |
|
1.13.11.60 | more |
sorbitol (0.75 M, 14%), glycerol (15%) and ethylene glycol (25%) do not inhibit the enzyme |
|
1.13.11.60 | more |
suicide inactivation, activity falls to zero within a few min although substrates were available in sufficient amounts |
|
1.13.11.60 | dithiothreitol |
- |
|
1.13.11.60 | Triton X-100 |
0.5%, inactivation |
|
1.13.11.60 | 1,10-phenanthroline |
2.5 mM, 50% inhibition |
|
1.13.11.60 | 2-mercaptoethanol |
- |
|
1.13.11.60 | glycine |
2% |
|