3.5.99.7 | 1-amino-2,2-difluorocyclopropane-1-carboxylic acid |
rac-DFACC, chemical synthesis, overview. Mechanism of decomposition and inhibition of 1-aminocyclopropane-1-carboxylic acid deaminase, and Michaelis-Menten kinetics. DFACC is unstable under near-physiological conditions where it primarily decomposes via specific-base catalysis to 3-fluoro-2-oxobut-3-enoic acid. Different decomposotion mechanisms are described. Upon incubation with ACC deaminase, DFACC is a slow-dissociating inhibitor of ACC deaminase with submicromolar affinity |
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