5.4.2.6 | after 12 h in 0.02 M imidazole buffer, at pH 7.0, totally inactivated both at 0°C and at -20°C. Glycylglycine, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and phosphate at the same conditions are able to preserve most of the enzyme activity. Cacodylate, ethylmorpholidate, Tris-maleate and glycerophosphate have an intermediate action |
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