EC Number   |
General Stability |
Reference |
|---|
    3.5.3.3 | addition of 0.2 M NaCl stabilizes against the solubilizing effect of urea. Methylurea has higher denaturing capacity than urea. At glycerol concentrations up to 20% creatinase is in its native state |
288994 |
| 3.5.3.3 | enzyme shows anomalously low intrinsic stability and is inaccessible to reconstitution after preceding deactivation, denaturation and dissociation |
288994 |
| 3.5.3.3 | stability of creatine amidinohydrolase-containing polyurethane hydrogels stored in buffer at 37°C decreases after 1 week, PEGylation of creatine-amidinohydrolase leads to decrease of half-life when stored in buffer at 37°C |
663469 |
| 3.5.3.3 | stabilized by addition of nonionic detergents with a hydrophile-lipophile balance value of more than 14, chelating agents, or preservatives |
288979 |
| 3.5.3.3 | unused enzyme in the immobilized creatininase-creatinase-sarcosine oxidase enzyme system maintains activity for at least 6 months. Activity remains unchanged after heavy daily use of 20-30 days |
288993 |
| 3.5.3.3 | upon increasing the concentration of the enzyme from 0.003 to 0.024 mg/ml the apparent concentration of guanidinium chloride where 50% denaturation are observed shifts from 0.35 to 0.44 M |
288994 |
