3.4.22.3 | ficin is threatened by two main factors, autolysis and aggregation. Autolysis of ficin in the absence of inhibitor is very large but in the presence of iodoacetamide it is completely inhibited. However ficin aggregation is greatly induced. This is because of beta-sheets increasing in the second structure, structure opening, ficin molecules adhesion to each other and molecules sedimentation. Iodoacetamide inhibits ficin activity irreversibility and aggregates large amount of protein. Therefore, iodoacetamide is not recommended for autolysis inhibition because of large aggregation. Tetrathionate has both activator and inhibitor effect on ficin autolysis at very low concentrations and higher concentrations respectively. Tetrathionate has a few side effects on structure opening, thermal stability decreasing and aromatic residues exiting. A tetrathionate inhibitory effect is reversible on ficin by dialyzing. |
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