EC Number   |
General Stability |
Reference |
|---|
   3.4.17.B1 | 10% glycerol stabilizes |
651034 |
| 3.4.17.B1 | 13% remaining activity in 0.1% SDS at 70°C after 180 min, stable at 40°C |
651034 |
| 3.4.17.B1 | 40% remaining activity in 8 M urea after 55 min at 40°C |
651034 |
| 3.4.17.B1 | after dilution of the carboxypeptidase in MES buffer, pH 6.5, in the absence of glycerol and 2-mercaptoethanol, the enzyme undergoes a slow loss of activity |
721897 |
| 3.4.17.B1 | immobilization of the enzyme on magnetic nanoparticles improves long-term stability at room temperature compared to the free native enzyme and also results in a significantly higher stability in organic solvents at 40°C |
729491 |
| 3.4.17.B1 | in the absence of glycerol and beta-mercaptoethanol, at 50°C, the enzyme undergoes a slow thermal inactivation upon dilution in an aqueous buffer at pH 6.5. This loss of activity can be inhibited when the enzyme is maintained at high pressure. At higher temperatures, higher pressures (up to 400 MPa) are required to maintain the enzyme in its active state. |
721897 |
| 3.4.17.B1 | inactivation is strongly temperature and pressure dependent |
721897 |
