EC Number   |
General Stability |
Reference |
|---|
    2.5.1.19 | complete unfolding of the protein at 2.5 M guanidine hydrochloride at pH 7.8 (midpoint guanidine hydrochloride concentration of unfolding: 0.8 +/-0.1 M), enhanced flexibility, lower stability and increased proteolysis of mutants A183T and G96A/A183T compared to wild-type and mutant G96A as revealed by susceptibility to proteolytic digestion with trypsin, thermolysin, pronase (25°C for various time intervals), unfolding profiles (intrinsic tryptophan fluorescence measurements at 340 nm) after treatment with guanidine hydrochloride (30 min at 25°C) and dynamic quenching with acrylamide or not, and secondary structure determination by circular dichroism spectroscopy |
691087 |
| 2.5.1.19 | dithiothreitol reverses aggregation which sometimes occurs after storage at -20°C |
638173 |
| 2.5.1.19 | dithiothreitol, 0.4 mM, stabilizes during purification |
638174 |
| 2.5.1.19 | EDTA stabilizes |
638181 |
| 2.5.1.19 | freezing inactivates, Polybuffer 74, operating range pH 7-4, 1 mM dithioerythritol stabilize |
638181 |
| 2.5.1.19 | glycerol, 50% v/v, stabilizes |
638173, 638174 |
| 2.5.1.19 | glyphosate-sensitive enzyme is more stable than wild-type enzyme |
638180 |
| 2.5.1.19 | prolonged dialysis against 50 mM Tris-HCl buffer, pH 7.5, 0.1 mM DTT, 1 mM benzamidine hydrochloride, 5 mM EDTA, 1 mM PMSF, stable to |
638185 |
