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EC Number General Stability Reference
Display the reaction diagram Show all sequences 2.4.1.231highly unstable during purification, glycerol, phosphate, dithiothreitol and PMSF stabilize 644907
Display the reaction diagram Show all sequences 2.4.1.231unstable protein, half-life of no more than approximately 1.3 h in 20mM MES buffer, 25°C, pH 7.0. The enzyme can be stabilized on addition of alpha,alpha-trehalose (300 mM) which yields a half-life of 11.5 h. In the presence of 20% (w/v) glycerol, and 9 and 26% (w/v) PEG-4000, half-life is 6.5 h, 16 h and 70 h, respectively. Large improvements in stability by covalent protein modification with activated derivatives of PEG-5000 674984
Results 1 - 2 of 2