EC Number   |
General Stability |
Reference |
|---|
    2.1.1.5 | divalent cations and 2-mercaptoethanol do not stabilize during purification |
441232, 441234 |
| 2.1.1.5 | glycerol does not stabilize |
441235 |
| 2.1.1.5 | lyophilization inactivates |
441232 |
| 2.1.1.5 | mechanism of unfolding by urea includes two intermediate states, a tetramer and a monomer. Dissociation is concomitant with alterations in the dimerization arm and the loop connecting the C-terminal helix, and intermediate conformations originate from perturbations in these structures |
657874 |
| 2.1.1.5 | partial digestion of ligand-free enzyme with trypsin produces two large peptides, excising a seven residue peptide with loop L2. Carboxybutylhomocysteine but not methionine slows proteolysis by trypsin |
657875 |
| 2.1.1.5 | substrates stabilize during purification |
441232, 441234 |
