EC Number   |
General Stability |
Reference |
|---|
    1.1.1.21 | -55°C, unstable to prolonged storage |
286246 |
| 1.1.1.21 | 25°C, 0.3 M phosphate or Tris, pH 6.0, more than 2 months |
286260 |
| 1.1.1.21 | alpha-crystallin stabilizes the enzyme with higher efficiency at 37°C compared to 25°C |
667331 |
| 1.1.1.21 | dithiothreitol, 2 mM stabilizes, other sulfhydryl compounds such as beta-mercaptoethanol, glutathione and cysteine are less effective |
286248 |
| 1.1.1.21 | DTT stabilizes the purified native enzyme from lens at 2 mM |
667224 |
| 1.1.1.21 | enzyme is not stable unless dithiothreitol in micromolar range is present |
656089 |
| 1.1.1.21 | extremely labile to freezing and thawing unless Carbowax 100 is included, 10 mg/ml |
286243 |
| 1.1.1.21 | freezing and thawing at temperatures above 50°C results in loss of activity |
286241 |
| 1.1.1.21 | freezing and thawing: more than twice, unstable |
286246 |
| 1.1.1.21 | freezing of purified enzyme results in complete loss of activity |
286186 |
