EC Number |
General Stability |
Reference |
---|
2.7.4.14 | bovine serum albumin, 0.1 mg/ml, is completely effective in preventing the loss of activity in the dilute preparation |
645137 |
2.7.4.14 | dialysis against 20 mM phosphate, 1 mM MgCl2, 20% ethylene glycol, pH 8.0, 90% loss of activity |
645147 |
2.7.4.14 | freeze-thawing inactivates |
645147 |
2.7.4.14 | more stable in histidine than in phosphate buffer |
645141 |
2.7.4.14 | the enzyme is unstable when fully activated, anions promoting hydrophobic interactions stabilize the active conformation |
645142 |
2.7.4.14 | the purified enzyme is notably unstable |
645141 |
2.7.4.14 | three-fold dilution of a preparation of 0.2 mg of protein per ml results in less of 50% of the activity in 1 h |
645137, 645140 |