EC Number |
General Stability |
Reference |
---|
1.8.4.2 | EDTA 5mM, 37°C, phosphate buffer, total loss of activity within 30-60 min, can partially be prevented by NADPH |
394815 |
1.8.4.2 | metal ions required for activity and maintenance of the proper conformation of the enzyme |
394815 |
1.8.4.2 | EDTA stabilizes during purification |
394820, 394848 |
1.8.4.2 | dimerization after prolonged storage at -20°C, freeze-thawing or heating at 60°C |
394836 |
1.8.4.2 | highly susceptible to proteolytic attack |
394844 |
1.8.4.2 | GSH, 10 mM, enhances heat stability |
394848 |
1.8.4.2 | insulin, 0.087 mM, decreases heat stability |
394848 |