EC Number |
General Stability |
Reference |
---|
1.4.1.13 | 2 mM alpha-ketoglutarate, 10 mM beta-mercaptoethanol and 10% glycerol when present together markedly enhance stability |
391440 |
1.4.1.13 | 2-mercaptoethanol, stabilizes |
391438, 391455 |
1.4.1.13 | alpha-ketoglutarate stabilizes |
391438, 391447, 391448 |
1.4.1.13 | alpha-ketoglutarate, L-glutamine, L-glutamate and NADP when present together partial protect against enzyme inactivation |
391438 |
1.4.1.13 | DTT, 1 mM, stabilizes at 4°C |
391455 |
1.4.1.13 | EDTA, stabilizes |
391448, 391455 |
1.4.1.13 | glutamine stabilizes |
391447, 391448 |
1.4.1.13 | incubation with L-glutamine or L-glutamate results in marked time- and temperature-dependent loss in activity |
391438 |
1.4.1.13 | NADP causes relatively small loss in activity compared with control values |
391438 |
1.4.1.13 | unprotected enzyme is unstable, losing 65% of its activity in 24 hours at 4°C |
391440 |