EC Number   |
General Stability  |
Reference |
|---|
   1.11.1.18 | activity half-life times increase along the series of buffers: phosphate <MES <Tris-HCl |
701152 |
| 1.11.1.18 | after treatment with a mixture of chloroform/ethanol the enzyme still shows 65% of its initial brominating activity |
675172 |
| 1.11.1.18 | Ca2+ significantly increased the enzyme stability. Strontium and magnesium ions have similar effects |
674916 |
| 1.11.1.18 | circular dichroism measurements showed that the secondary structure is not affected upon incubation in 4% SDS |
672310 |
| 1.11.1.18 | denaturation did not occur upon incubation in 4 M guanidine hydrochloride |
672310 |
| 1.11.1.18 | dialysis of the enzyme preparation against 1 mM EDTA in 0.1 M citrate-phosphate buffer (pH 3.8) results in loss of enzymic activity. Incubation with vanadium, does not restore the enzymic activity. Also various other metal ions: Zn(II), Fe(II), Cu(II) and Mn(II) are ineffective in the reactivation of the preparation |
672433 |
| 1.11.1.18 | vanadate increases the thermostability |
674916 |
