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Results 1 - 7 of 7
EC Number
General Stability
activity half-life times increase along the series of buffers: phosphate <MES <Tris-HCl
after treatment with a mixture of chloroform/ethanol the enzyme still shows 65% of its initial brominating activity
Ca2+ significantly increased the enzyme stability. Strontium and magnesium ions have similar effects
circular dichroism measurements showed that the secondary structure is not affected upon incubation in 4% SDS
denaturation did not occur upon incubation in 4 M guanidine hydrochloride
dialysis of the enzyme preparation against 1 mM EDTA in 0.1 M citrate-phosphate buffer (pH 3.8) results in loss of enzymic activity. Incubation with vanadium, does not restore the enzymic activity. Also various other metal ions: Zn(II), Fe(II), Cu(II) and Mn(II) are ineffective in the reactivation of the preparation
vanadate increases the thermostability
Results 1 - 7 of 7