EC Number   |
Activating Compound |
Reference |
|---|
   3.6.4.B7 | ADP |
activates binding of ssDNA |
756571 |
| 3.6.4.B7 | more |
no DNA binding is detected in the absence of nucleotide (ADP), but also no DNA binding is detected with ATP or dATP or in the presence of poorly-hydrolyzable ATP analogues, ATPgammaS or AMP-PNP |
756571 |
| 3.6.4.B7 | more |
RadC1 and RadC2 are involved in DNA repair |
733620 |
| 3.6.4.B7 | Rad54 protein |
the Rad54 protein is a double-strand DNA-dependent ATPase that can alter the topology of duplex DNA. Like its eukaryotic homolog, it interacts directly with the SsoRadA, to stimulate DNA strand exchange |
726065 |
| 3.6.4.B7 | RadC1 |
Sto0579, enhances the ATPase and strand invasion activities of RadA |
733620 |
| 3.6.4.B7 | RadC2 |
Sto1830, interacts with both RadA and Hjc, a Holliday junction resolvase |
733620 |
| 3.6.4.B7 | RecA |
RecA interacts with and loads wild-type RadA to promote unwinding of a non-cognate 3'-tailed or 5'-fork DNA substrate and of a 5'-invading D-loop. Wild-type RadA interaction with RecA is crucial to recruit the former onto D-loop DNA, and both proteins in concert catalyse D-loop extension to favour integration of ssDNA during chromosomal transformation. Proposed model for the action of the 5'->3' RadA helicase in coordination with RecA during natural transformation and in doublee strand break repair, overview. RecA has ATPase activity, but RadA inhibits the ATPase activity of RecA. Mutational function analysis, overview |
757864 |
| 3.6.4.B7 | RecA |
RecA is required for RadA-stimulation of strand exchange. RecA can also catalyze strand-exchange between two duplex molecules, provided that strand exchange is initiated at a short ssDNA gap in the substrate. These reactions produce full 4-strand Holliday junction intermediates |
756571 |
| 3.6.4.B7 | replication protein A |
stimulates the RadA promoted strand-exchange reaction in vitro |
725343 |
| 3.6.4.B7 | single-stranded DNA |
ssDNA, like RecA, RadA's ATPase activity is strongly stimulated by ssDNA |
756571 |
