EC Number   |
Activating Compound |
Reference |
|---|
   3.4.22.2 | 2-mercaptopropionic acid |
required, complete activation at 0.05 N when the enzyme concentration is 0.49 mM |
95693 |
| 3.4.22.2 | Cys |
activates |
95663, 95669, 95678 |
| 3.4.22.2 | cysteine |
the requirement for reducing conditions during the activation process is investigated with 20 mM DTT or 20 mM cysteine, propapain does not convert to papain even after prolonged incubation with DTT, whereas in presence of 20 mM cysteine, the activation occurs between 30 and 40 min at 50°C |
700680 |
| 3.4.22.2 | EDTA |
required for maximal activity |
95663 |
| 3.4.22.2 | glutathione |
- |
95678 |
| 3.4.22.2 | Hg2+ |
papain activity increases to a maximum of 111.03% (non-competitive type activation) at a concentration of 0.000001x01mol/l Hg2+, but is almost completely deactivated at concentrations above 0.0001 mol/lx01Hg2+. The inhibition of Hg2+ on papain is a competitive and uncompetitive mixed type inhibition |
755374 |
| 3.4.22.2 | more |
alkylation of a cysteine residue in papain with a pyridoxamine results in a semi-synthetic enzyme papain-PX that has no detectable protease activity but has the ability to catalyze enantioselective reductive amination of alpha-keto acids such as alpha-ketoglutarate and pyruvate. Papain-PX reductively aminates the alkyl side chain of functionalized alpha-keto acids to give the respective alpha-amino acids with enantioselectivities greater than 70% |
707460 |
| 3.4.22.2 | more |
loosely packed papain prosegment displays inhibitory activity but can also function as activator for the mature enzyme, overview |
667324 |
| 3.4.22.2 | Reducing agents |
e.g. 2,3-dimercaptopropanol, activates |
95663 |
| 3.4.22.2 | Sodium borohydride |
activates |
95663 |
