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EC Number
Activating Compound
Commentary
Reference
3.2.1.81
2-mercaptoethanol
30% increase in activity
677787
3.2.1.81
beta-mercaptoethanol
beta-mercaptoethanol even at a low concentration of 10 mM increases the activity of AgaA by 23%
705482
3.2.1.81
CaCl2
stimulation
678815
3.2.1.81
dithiothreitol
30% increase in activity
677787
3.2.1.81
dithiothreitol
dithiothreitol even at a low concentration of 10 mM increases the activity of AgaA by 21%
705482
3.2.1.81
EDTA
reverses inhibition by bivalent metal cations
136888
3.2.1.81
EDTA
stabilizes enzyme
669065
3.2.1.81
more
no activation of rAgaB34 by chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea)
699497
3.2.1.81
NaCl
145% activity at 500 mM NaCl
714561
3.2.1.81
NaCl
maximum activity in presence of 2% NaCl
667288
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