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EC Number	Activating Compound	Commentary	Reference
3.1.26.4	(NH4)2SO4	100 mM, isoenzyme I and II are activated by 50%. Isoenzyme II shows optimal activity at 50 mM, 80% inhibition at 200 mM	134390
3.1.26.4	(NH4)2SO4	30% activation of Mg2+-dependent enzyme	134394
3.1.26.4	(NH4)2SO4	4fold stimulation at an optimal concentration of 18 mM	134397
3.1.26.4	(NH4)2SO4	optimal concentration: 25 mM	134387
3.1.26.4	(NH4)2SO4	ribonuclease H I acts optimally at about 30 mM (NH4)2SO4 in the case of Mn2+ activation, in the presence of Mg2+ ions the enzyme acts best if (NH4)2SO4 is omitted	134371
3.1.26.4	2-mercaptoethanol	-	656122
3.1.26.4	2-mercaptoethanol	enzyme form H2 is inactive in absence of mercaptoethanol. No effect on enzyme form H1	134399
3.1.26.4	2-mercaptoethanol	stimulates	134397
3.1.26.4	dithiothreitol	optimal concentration is 1 mM	134077
3.1.26.4	DTT	-	656122

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Release 2023.1 (January 2023)