EC Number |
Activating Compound |
Reference |
---|
2.5.1.20 | 2-mercaptoethanol |
10 mM, not as effective as dithiothreitol |
638726 |
2.5.1.20 | dithiothreitol |
10 mM, required for maximal stability |
638726 |
2.5.1.20 | EDTA |
approx. 3.5fold activation at 20 mM |
638736 |
2.5.1.20 | glutathione |
required for maximal activity |
638730, 638731 |
2.5.1.20 | more |
recombinant HRT2, expressed in Escherichia coli, is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber, but the recombinant HRT2 does not exhibit significant activity independently. HRT1/HRT2 require additional cofactors from the eukaryotic cells to produce distinct cPT activity, which seem to be contained in latex species |
723380 |
2.5.1.20 | neryl diphosphate |
accelerates activity |
638725 |
2.5.1.20 | Rubber elongation factor |
amino acid sequence of REF |
638729 |
2.5.1.20 | Rubber elongation factor |
i.e.REF, protein tightly bound to serum-free rubber particles, required for prenyltransferase activity, no requirement for farnesyl diphosphate synthase activity |
638728 |
2.5.1.20 | rubber particle |
- |
638730 |
2.5.1.20 | sulfhydryl compound |
essential for full activity |
638725 |