   3.4.21.90 | more |
cores, which are resistant to trypsin at physiological salt concentrations, whereas become trypsin sensitive at elevated salt concentrations and under these conditions, the carboxy-terminal domain, beginning with position M(106) is released as a soluble protein. Use of endoproteinase LysC instead of trypsin allows a somewhat larger soluble carboxy-terminal domain beginning with Q(94). The disassembly of cores is stimulated by increasing the concentration of NaCl in the reaction, by the presence of octylglycoside, and by lowering the pH of the reaction to 6.0 and is not stimulated by the presence of NP 40 |
695938 |
