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EC Number	Activating Compound	Commentary	Reference
3.2.1.123	more	for maximal hydrolysis of glycosphingolipids by rhodococcal EGCases, a detergent such as Triton X-100 is required at a final concentration of 0.1-0.3% of the reaction mixture. The hydrolysis of water soluble gangliosides such as GM1 does not require the presence of a detergent. For the hydrolysis of GM1, the addition of 5% tetrahydrofuran instead of Triton X-100 increases the reaction rate of EGCase I by 2fold compared to that in the absence of a detergent. Tetrahydrofuran is ineffective in facilitating the hydrolysis of GbOse4Cer by EGCase I. DMSO, ethyl acetate, and methyl cellosolve are also not effective in stimulating the hydrolysis of GM1 or GbOse4Cer by EGCase I in the absence of a detergent	698135
3.2.1.123	Nonidet P-40	activates	646691
3.2.1.123	Nonidet P-40	activates at 0.2%	646688
3.2.1.123	sodium cholate	-	646691
3.2.1.123	sodium taurodeoxycholate	activates endoglycoceramidase I and II, inhibition of endoglycoceramidase I above 0.4% w/v	646691
3.2.1.123	taurodeoxycholate	0.5 mg/ml, 2.5fold increase of activity	646687
3.2.1.123	tetrahydrofuran	the activity is enhanced by addition of 5% (v/v) tetrahydrofuran	732295
3.2.1.123	Triton X-100	0.2% (w/v)	665547
3.2.1.123	Triton X-100	activates	646691
3.2.1.123	Triton X-100	activates at 0.2%	646688, 646695

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Release 2023.1 (January 2023)