6.3.2.7 A409R site-directed mutagenesis, the lytic phenotype observed upon overexpression of the wild-type murESa gene is replicated with the mutant 727964 6.3.2.7 D406A site-directed mutagenesis, the mutant shows very low or no activity in vivo 727964 6.3.2.7 E460A site-directed mutagenesis, the mutant shows very low or no activity in vivo 727964 6.3.2.7 N407A site-directed mutagenesis, inactive mutant 727964 6.3.2.7 N407R site-directed mutagenesis, the growth curve of N407R mutant is similar to that of wild-type murESa 727964 6.3.2.7 P408A site-directed mutagenesis, the lytic phenotype observed upon overexpression of the wild-type murESa gene is replicated with the mutant 727964