6.2.1.3 C343S no luminescence enhancement 697221 6.2.1.3 C343S/G344T no luminescence enhancement 697221 6.2.1.3 C343S/G344T/L345S increase of luminescence activity to 100% 697221 6.2.1.3 C343S/L345S increase of luminescence activity 697221 6.2.1.3 E361A inactive mutant enzyme 652285 6.2.1.3 F319A site-directed mutagenesis 714605 6.2.1.3 F319W site-directed mutagenesis 714605 6.2.1.3 F319Y site-directed mutagenesis 714605 6.2.1.3 G216A the ratio of turnover number to Km-value for the reaction with oleate is 32.7% of the wild-type ratio 652285 6.2.1.3 G219A the ratio of turnover number to Km-value for the reaction with oleate is 1.3fold higher than the wild-type ratio 652285