5.4.99.39 C262S mutation in region responsible for product differentiation. Like wild-type, mutant produces beta-amyrin as sole product 683581 5.4.99.39 C486A mutant shows 46% activity compared to wild-type (100%), Km similar to wild-type, kcat decreased 729707 5.4.99.39 C564A mutant shows 1.6% activity compared to wild-type (100%), Km increased compared to wild-type, kcat highly decreased 729707 5.4.99.39 D485E mutant shows no activity 729707 5.4.99.39 D485N mutant shows no activity 729707 5.4.99.39 F413A site-directed mutagenesis, functional analysis, the mutant shows slightly reduced activity compared to thre wild-type enzyme 748812 5.4.99.39 F413H site-directed mutagenesis, functional analysis, the mutant shows slightly increased activity compared to thre wild-type enzyme 748812 5.4.99.39 F413M site-directed mutagenesis, functional analysis, the mutant shows slightly reduced activity compared to thre wild-type enzyme 748812 5.4.99.39 F413S site-directed mutagenesis, functional analysis, the mutant shows slightly increased activity compared to thre wild-type enzyme 748812 5.4.99.39 F413T site-directed mutagenesis, functional analysis, the mutant shows slightly increased activity compared to thre wild-type enzyme 748812