4.4.1.16	C364A	increased activity towards L-selenocysteine and L-cysteine sulfinate, activity toward L-cysteine was completely abolished	645632	
4.4.1.16	C375A	mutation on the catalytically essential Cys-375 residue	704655	
4.4.1.16	D146K	site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity	730685, 730686	
4.4.1.16	D146K/H389T	site-directed mutagenesis,the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity to a higher extent	730685, 730686	
4.4.1.16	H123A	only 1% of activity remaining compared to wild type, contributes to stabilization of pyridoxal 5'-phospahte	645630	
4.4.1.16	H389T	site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity	730686	
4.4.1.16	H55A	no effect on activity	645630	
4.4.1.16	additional information	construction of enzyme knockout mice, phenotype, overview	730381	
4.4.1.16	additional information	HeLa cells transfected with enzyme-specific siRNA show decreased seleno-labeling efficiency of selenoproteins with selenoprotein P compared to the wild-type	730241	
4.4.1.16	R379A	less than 1% of activity remaining compared to wild type	645630