4.2.3.108	F266C	site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme	748984	
4.2.3.108	F266S	site-directed mutagenesis, the mutation shifts the the product spectrum significantly toward alpha-terpineol compared to wild-type enzyme	748984	
4.2.3.108	F266T	site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme	748984	
4.2.3.108	F266V	site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme	748984	
4.2.3.108	F266Y	site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme	748984	
4.2.3.108	additional information	although the distributions of the five detectable products sabinene, beta-myrcene, limonene, 1,8-cineole, and alpha-terpineol change slightly in all these mutants compared to wild-type, none of them shifts the product spectrum significantly toward alpha-terpineol, with exception of mutant F266S, overview	748984	
4.2.3.108	additional information	conversion of CinS1 to a sabinene synthase based on an amino acid comparison of the enzyme with Salvia officinalis SabS1 and Salvia pomifera SabS1	718210	
4.2.3.108	additional information	exchange of sequences corresponding by homology to the C-terminal domain including the domain spanning alpha-helix, between sabinene synthase and both bornyl diphosphate synthase and cineole synthase. Exchange of residues 304-377 from cineole synthase into sabinene synthase is sufficient to impart the alternative termination chemistry involving water capture to alpha-terpineol and cyclization to 1,8-cineole	717153	
4.2.3.108	additional information	the N-terminal signal peptide of AaTPS6 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3)	747873	
4.2.3.108	N338A	products are 11.7% alpha-pinene, 33.1% sabinene, 33.2% beta-pinene, 16.9% myrcene, 5.1% limonene	718210