4.2.1.51	C216A	site-directed mutagenesis, inactive mutant	649980	
4.2.1.51	C216S	site-directed mutagenesis, increased activity	649980	
4.2.1.51	E159A	site-directed mutagenesis, 2.2fold increased activity	649980	
4.2.1.51	E159A/E232A	site-directed mutagenesis, 7fold increased kcat, 4.6fold decreased Km compared to the wild-type, increased activity	649980	
4.2.1.51	E232A	site-directed mutagenesis, 3.5fold increased activity	649980	
4.2.1.51	E64D	constructed by PCR-based random mutagenesis and complementation analysis, 15% reduced activity compared to the wild-type enzyme, 4.5% increased Km, 1.7fold increased kcat	649516	
4.2.1.51	E64Q	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	E64S	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	E64V	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	F185L	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516