4.2.1.143 additional information aflK mutant construction by gene replacement. The aflK deletion-mutant DELTAaflK exhibits a significant decrease in sclerotial production and aflatoxin biosynthesis compared with wild-type and the complementation strain DELTAaflK::aflK. The mutation DELTAaflK does not affect the ability of Aspergillus flavus to infect seeds, but downregulates aflatoxin production after seed infection -, 748620 4.2.1.143 additional information truncation of the N-terminal 41-amino-acid region may not affect the substrate specificity -, 721335