4.2.1.125	C264A	site-directed mutagenesis, the mutant shows 53% decreased activity compared to the wild-type enzyme	748118	
4.2.1.125	C489A	site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme	748118	
4.2.1.125	C568A	site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme	748118	
4.2.1.125	D488A	site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme	748118	
4.2.1.125	F477A	site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme	748118	
4.2.1.125	I559A	site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme	748118	
4.2.1.125	additional information	establishment of dammarane-type ginsenosides synthesis from 2,3-oxidosqualene in Oryza sativa by expression of dammarenediol-II synthase from Panax ginseng resulting in a dammarane-type sapogenin 20(S)-protopanaxadiol (PPD) content of 0.35-0.59 mg/gdw and a dammarane-type sapogenin 20(S)-protopanaxatriol (PPT) content of 0.23-0.43 mg/g dry weight in the transgenic rice plants	749016	
4.2.1.125	additional information	expression in Saccharomyces cerevisiae mutant lacking lanosterol synthase results in production of dammarenediol and hydroxydammarenone. Silencing of gene expression by RNAi leads to a reduction of ginsenoide production to 84.5% in roots	683979	
4.2.1.125	additional information	functionally expressed in the lanosterol synthase-deficient yeast mutant erg7	710295	
4.2.1.125	additional information	heterologous biosynthesis of triterpenoid dammarenediol-II, the precursor of dammarane-type tetracyclic ginsenosides, in engineered Escherichia coli strain BL21(DE3) by expressing the enzyme, and coexpressing squalene synthase, squalene epoxidase, and NADPH-cytochrome P450 reductase from Saccharomyces cerevisiae, and squalene epoxidase from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana, and thereby reconstituting the 2,3-oxidosqualene-derived triterpenoid biosynthetic pathway. Method evaluation and optimization, overview	747390